A study of the constitutive heterochromatin and nucleolus organizer regions of Isocopris inhiata and Diabroctis mimas (Coleoptera: Scarabaeidae, Scarabaeinae) using C-banding, AgNO3 staining and FISH techniques
Genet. mol. biol
; 28(1): 111-116, Jan.-Mar. 2005. ilus
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| ID: lil-399625
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BR26.1
RESUMO
Meiotic and mitotic chromosomes of Isocopris inhiata and Diabroctis mimas were studied by standard staining procedures, C-banding, silver nitrate staining and FISH using Apis mellifera 28S rDNA as probe. Isocopris inhiata presented a 2n = 18 (8II+ Xy p) karyotype, composed of meta-submetacentric chromosomes with gradual reduction in size. The karyotype of D. mimas was 2n = 20 (9II+ Xy p), composed of meta-submetacentric (pairs 1, 2, 3, 4 and 7) and acrocentric (pairs 5, 6, 8 and 9) chromosomes, with gradual reduction in size. Analysis of constitutive heterochromatin revealed similar C-banding patterns for both species, showing pericentromeric and telomeric bands and diphasic chromosomes. In addition, the X chromosomes of these species were found to be almost completely heterochromatic. The presence of chromocenters was checked in one or more phases of prophase I of these species. All heterochromatin reacted positively for the silver stain. By FISH analysis we were able to locate the rDNA in medium-size autosome pairs in both species and in the X chromosome of D. mimas.
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Índice:
LILACS
Asunto principal:
Escarabajos
/
ADN Ribosómico
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Heterocromatina
Límite:
Animals
Idioma:
En
Revista:
Genet. mol. biol
Asunto de la revista:
GENETICA
Año:
2005
Tipo del documento:
Article