Typing class I HLA-A gene using a nested PCR-RFLP procedure
Braz. j. med. biol. res
;
38(6): 837-842, June 2005. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-402664
ABSTRACT
In order to detect several new HLA-A class I alleles that have been described since 1998, the original PCR-RFLP method developed to identify the 78 alleles recognized at that time at high resolution level was adapted by us for low and medium resolution levels using a nested PCR-RFLP approach. The results obtained from blood samples of 23 subjects using both the PCR-RFLP method and a commercial kit (MicroSSP1A®, One Lambda Inc.) showed an agreement higher than 95 percent. The PCR-RFLP adapted method was effective in low and medium resolution histocompatibility evaluations.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Prueba de Histocompatibilidad
/
Genes MHC Clase I
/
Antígenos HLA-A
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
Límite:
Humanos
Idioma:
Inglés
Revista:
Braz. j. med. biol. res
Asunto de la revista:
Biologia
/
Medicina
Año:
2005
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade Estadual Paulista/BR
/
Universidade de São Paulo/BR
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