RNA fingerprinting using RAP-PCR identifies an EBAF homologue mRNA differentially expressed in rat oviduct
Biocell
;
28(3): 287-297, dic. 2004. ilus
Artículo
en Inglés
| LILACS
| ID: lil-405201
ABSTRACT
As a step towards the identification of genes preferentially expressed in the oviduct during early rat embryo development, we isolated a cDNA fragment (Pr14) by using RNA arbitrarily primed PCR (RAP-PCR), being its expression restricted to oviduct and uterus; its mRNA is mainly expressed in oviduct during late luteal phase and early pregnancy. This fragment is 100 per cent identical to a rat DNA sequence (Accession No. NW_047400)downstream the terminal exon of a Ratturs norvegicus gene (Locus Link Accession No. LOC289316) similar to ebaf (endometrial bleeding-associated factor), a novel member of the Transforming Growth Factor superfamily. Northern analyses showed that this sequence hybridizes with 2.9 kb and 4.1 kb mRNAs in early pregnant rat oviducts. However, only the 4.1 kb mRNA was detected in the oviduct of non-pregnant rats, showing an increase from proestrus to diestrus. The expression of this oviduct-uterus specific mRNA suggests that the products of this gene may play a role in the oviductal reproductive process.
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Índice:
LILACS (Américas)
Asunto principal:
Factores de Transcripción
/
ARN Mensajero
/
Perfilación de la Expresión Génica
/
Trompas Uterinas
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Biocell
Asunto de la revista:
Clulas
Año:
2004
Tipo del documento:
Artículo
País de afiliación:
Argentina
Institución/País de afiliación:
Universidad Nacional de Tucumán/AR
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