Analysis of expressed sequence tags from Trypanosoma cruzi amastigotes

ABSTRACT

A total of 880 expressed sequence tags (EST) originated from clones randomly selected from a Trypanosoma cruzi amastigote cDNA library have been analyzed. Of these, 40 percent (355 ESTs) have been identified by similarity to sequences in public databases and classified according to functional categorization of their putative products. About 11 percent of the mRNAs expressed in amastigotes are related to the translational machinery, and a large number of them (9 percent of the total number of clones in the library) encode ribosomal proteins. A comparative analysis with a previous study, where clones from the same library were selected using sera from patients with Chagas disease, revealed that ribosomal proteins also represent the largest class of antigen coding genes expressed in amastigotes (54 percent of all immunoselected clones). However, although more than thirty classes of ribosomal proteins were identified by EST analysis, the results of the immunoscreening indicated that only a particular subset of them contains major antigenic determinants recognized by antibodies from Chagas disease patients.