Detection of hepatitis C virus RNA by in situ hybridization in paraformaldehyde fixed biopsies
Mem. Inst. Oswaldo Cruz
;
100(3): 269-272, May 2005. ilus
Artículo
en Inglés
| LILACS
| ID: lil-411022
ABSTRACT
Fourteen hepatitis C virus (HCV) chronically infected patients were submitted to routine liver biopsy for histological evaluation. Liver samples were assayed to HCV-RNA by in situ hybridization, using digoxigenin labeled probe. HCV genotypes were found to be predominantly type 1 (71.4 percent), followed by genotype 3 (21.4 percent), and genotype 2 (7.2 percent). Alanine-aminotransferase levels were raised in 10 patients. The histopathological scores were minimal (21.4 percent), mild (57.2 percent), and moderate (21.4 percent). Viral RNA was detected in liver cells from nine patients (64.3 percent). ISH method provides localization and poor confirmation of HCV RNA in the liver tissue of HCV chronic patients.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
ARN Viral
/
Hibridación in Situ
/
Hepacivirus
/
Hepatitis C Crónica
/
Hígado
Tipo de estudio:
Estudio diagnóstico
Límite:
Adulto
/
Anciano
/
Femenino
/
Humanos
/
Masculino
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2005
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Fiocruz/BR
/
Universidade Federal do Rio de Janeiro/BR
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