Immunization with native and cobalt 60-irradiated Crotalus durissus terrificus venom in swiss mice: assessment of the neutralizing potency of antisera
J. venom. anim. toxins incl. trop. dis
;
11(3): 299-314, jul.-set. 2005. tab, graf
Artículo
en Inglés
| LILACS
| ID: lil-414927
ABSTRACT
ELISA was used to evaluate, follow, and compare the humoral immune response of Swiss mice during hyperimmunization with natural and Cobalt 60-irradiated (60Co) Crotalus durissus terrificus venom. Potency and neutralization were evaluated by in vitro challenges. After hyperimmunization, immunity was observed by in vivo challenge and the side effects were assessed. The animals immunization with one LD50 of the venom was on days one, 15, 21, 30, and 45, when blood samples were collected; the challenges occurred on the 60th day. Results showed that ELISA was efficient in evaluating, following, and comparing mouse immune response during hyperimmunization. Serum titers produced with natural venom were similar to those produced with irradiated venom. Immunogenic capacity was maintained after 60Co irradiation. Serum produced from Crotalus durissus terrificus irradiated venom showed higher potency and neutralization capacity than that from natural venom. All antibodies were able to neutralize five LD50 from these venoms. Clinical alterations were minimum during hyperimmunization with irradiated venom
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Inmunización
/
Cobalto
/
Venenos de Crotálidos
/
Ratones
Límite:
Animales
Idioma:
Inglés
Revista:
J. venom. anim. toxins incl. trop. dis
Asunto de la revista:
Toxicología
Año:
2005
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
CNEN
/
Sao Paulo State University/BR
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