Influence of culture conditions on Vero cell propagation on non-porous microcarriers
Braz. arch. biol. technol
;
48(spe): 71-77, June 2005.
Artículo
en Inglés
| LILACS
| ID: lil-415459
RESUMO
Animal cell cultures are widely employed for the production of viral vaccines and for recombinant protein expression. The cell line Vero is a continuous, adherent cell line, which has been recommended by the World Health Organization for the production of human vaccines. For the large-scale production of vaccines, microcarriers, which are microspheres that serve as support for the cells, are being increasingly used. The use of microcarriers in stirred bioreactors allows high cell densities and, consequently, high virus titres to be achieved. With the aim of selecting appropriate culture conditions for the cultivation of Vero cells at high cell densities, in this work the influence of several variables (agitation rate, ratio of inoculated cells to microcarrier mass and fetal bovine serum concentration) on cell growth on Cytodex 1 microcarriers was studied. Under the best conditions determined, a comparison with Vero cell cultivation on Cytodex 3 microcarriers was carried out.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Idioma:
Inglés
Revista:
Braz. arch. biol. technol
Asunto de la revista:
Biologia
Año:
2005
Tipo del documento:
Artículo
/
Congreso y conferencia
País de afiliación:
Brasil
Institución/País de afiliación:
FIOCRUZ/BR
/
Universidade Federal do Rio de Janeiro/BR
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