Development of bovine embryos reconstructed by nuclear transfer of transfected and non-transfected adult fibroblast cells
Genet. mol. res. (Online)
;
4(1): 55-66, Mar. 2005.
Artículo
en Inglés
| LILACS
| ID: lil-417409
ABSTRACT
An association of two techniques, nuclear transfer (NT), and transfection of somatic animal cells, has numerous potential applications and considerable impact, mainly in agriculture, medicine, pharmacy, and fundamental biology. In addition, somatic cell nuclear transfer is the most efficient alternative to produce large transgenic animals. We compared in vitro and in vivo developmental capacities of NT using fibroblast cells isolated from a 14-month-old cloned Simmental heifer (FCE) vs the same line transfected with a plasmid containing neomycin-resistant genes (TFCE). There were no significant differences (P > 0.5) in either fusion (116/149 = 78% vs 216/301 = 72%), cleavage (78/116 = 67% vs 141/216 = 65%) and blastocyst (35/116 = 30% vs 52/216 = 24%) rates or in pregnancy rate at 30 to 35 days after embryo transfer (2/17 vs 3/17) between NT using FCE and TFCE, respectively. Transfection and long-term in vitro culture of transfected cells did not affect developmental capacity of NT embryos up to 40 days of gestation
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Bovinos
/
Animales Modificados Genéticamente
/
Núcleo Celular
/
Transferencia de Embrión
/
Fibroblastos
Límite:
Animales
/
Embarazo
Idioma:
Inglés
Revista:
Genet. mol. res. (Online)
Asunto de la revista:
Biologia Molecular
/
Genética
Año:
2005
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Embrapa Genetic Resources and Biotechnology/BR
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