Intracellular polyamine pools, oligopeptide-binding protein A expression, and resistance to aminoglycosides in Escherichia coli
Mem. Inst. Oswaldo Cruz
;
100(7): 789-793, Nov. 2005. tab, graf
Artículo
en Inglés
| LILACS
| ID: lil-419708
RESUMO
The role of intracellular free polyamine (putrescine and spermidine) pools in multiple resistance to aminoglycoside antibiotics was investigated among in vitro selected kanamycin-resistant Escherichia coli J53 mutants expressing diminished oligopeptide-binding protein (OppA) levels and/or defective ornithine decarboxylase (ODC) activity. The results suggest that diminished OppA content, but not defective ODC activity expression, increased the relative concentration of free spermidine as compared to the wild type strain. Moreover, by adding exogenous polyamines or polyamine synthesis inhibitors to cultures with different mutant strains, a direct relationship between the intracellular OppA levels and resistance to kanamycin was revealed. Collectively these results further suggest a complex relation among OppA expression, aminoglycoside resistance and polyamine metabolism.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Poliaminas
/
Proteínas Bacterianas
/
Farmacorresistencia Bacteriana Múltiple
/
Escherichia coli
/
Aminoglicósidos
/
Antibacterianos
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2005
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade Católica de Santos/BR
/
Universidade de São Paulo/BR
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