Quantification of 4-nerolidylcatechol from Pothomorphe umbellata (Piperaceae) in rat plasma samples by HPLC-UV
RBCF, Rev. bras. ciênc. farm. (Impr.)
;
40(3): 373-380, jul.-set. 2004. ilus, tab, graf
Artículo
en Inglés
| LILACS
| ID: lil-420685
RESUMO
The oral dosages formulations from Pothomorphe umbellata (Piperaceae) can only be therapeutically evaluated after establishing 4-nerolidylcatechol (4-NRC) pharmacokinetic profile in plasma. For that purpose, a unique analytical method validation using HPLC-UV detection was developed for analysis of rat plasma samples. The animals received 10 mg/kg b.w. of 4-NRC i.v.. Analytical conditions were set with C18 column, methanol acetonitrile water (622018) as mobile phase, and a flow rate of 1.6 mL/min. The assay was linear from 1.0 - 80.0 mcg/mL. The recovery procedure was performed by liquid-liquid extraction showing quantitative extraction (106.4 ± 8.7 por cento)...
Texto completo:
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Índice:
LILACS (Américas)
Asunto principal:
Plantas Medicinales
/
Tecnología Farmacéutica
/
Medicina de Hierbas
Límite:
Animales
Idioma:
Inglés
Revista:
RBCF, Rev. bras. ciênc. farm. (Impr.)
Asunto de la revista:
Bioquímica
/
Farmacia
/
Farmacología
Año:
2004
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade de São Paulo/BR
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