Digoxigenin-labeled probe for rabies virus nucleoprotein gene detection
Rev. Soc. Bras. Med. Trop
;
39(2): 159-162, mar.-abr. 2006. ilus, tab
Artículo
en Inglés
| LILACS, SES-SP
| ID: lil-426908
ABSTRACT
A digoxigenin-labeled probe was produced from the Pasteur virus strain for the detection of the rabies virus N gene. The probe hybridization was performed from amplified N gene obtained by reverse transcription polymerase chain reaction and the results by RT-PCR and hybridization showed 100 percent agreement. The hybridization, when carried out in products amplified by RT-PCR, increases the sensitivity of this technique even more and confers specificity to the diagnosis. The technique described in this work will be useful in rabies diagnosis laboratories, once the cost is compatible with traditional rabies diagnostic techniques.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Rabia
/
Virus de la Rabia
/
ADN Viral
/
Digoxigenina
/
Nucleoproteínas
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Rev. Soc. Bras. Med. Trop
Año:
2006
Tipo del documento:
Artículo
Institución/País de afiliación:
Institute Pasteur São Paulo/BR
/
University of São Paulo/BR
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