Comparative study of DNA synthesis and nucleolar organizer regions of sinusoid littoral cells in mouse regenerating liver
Biocell
;
29(1): 33-38, abr. 2005. ilus, tab, graf
Artículo
en Inglés
| LILACS
| ID: lil-429664
ABSTRACT
Variations in DNA synthesis (DNAs) and Nucleolar Organizer Regions (NORs) were studied in the littoral cell population from regenerating liver of C3HS inbred mice standardized for periodicity analysis.Immunohistochemieal detection of Bromodeoxyuridine (BrdU) with a monoclonal antibody and silver staining of NORs (AgNORs) were assessed by means of a digital image analysis system in histological sections. Tissue samples were obtained every four hours from the 30th to the 54th hours after a partial hepatectomy. The results showed, in both parameters, a gradual increment of the values during the period studied, with highest values (DNAs 107.1 ± 16.1 SE; AgNORs 77. 3± 3.4 SE) located at 1600/54 Time of Day / Hours Post Hepatectomy (TD/HPH), which were significantly different (p <0.001) from the values of the first sample (DNAs 38.1 ± 9.5 SE; AgNORs 27.3 ± 1.0 SE) taken at 1600/30 TD/HPH. The results of our experiment demonstrate the existence of a strong correlation of DNA synthesis measured by BrdU immunohistoehemistry andAgNORs numbers in sinusoid littoral cells from mouse regenerating liver
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Índice:
LILACS (Américas)
Asunto principal:
Regeneración
/
Bromodesoxiuridina
/
ADN
/
Inmunohistoquímica
/
Hepatocitos
/
Hepatectomía
/
Hígado
Límite:
Animales
Idioma:
Inglés
Revista:
Biocell
Asunto de la revista:
Clulas
Año:
2005
Tipo del documento:
Artículo
País de afiliación:
Argentina
Institución/País de afiliación:
Universidad Nacional de La Plata/AR
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