Heme oxygenase 1 overexpression increases iron fluxes in Caco-2 cells
Biol. Res
;
39(1): 195-197, 2006. ilus
Artículo
en Inglés
| LILACS
| ID: lil-430714
ABSTRACT
Heme oxygenase-1 is a microsomal enzyme that, when induced by stress, protects the cells from oxidative injury. Heme oxygenase-1 participates in the cleavage of the heme ring producing biliverdin, CO and ferrous Fe. The released Fe becomes part of intracellular Fe pool and can be stored in ferritin or released by an iron exporter. The mechanism by which heme enters cells is not completely understood, although it had been suggested that it might be internalized by an endocytosis process. In this study, we expressed a full-length Heme oxygenase-1 cDNA in Caco-2 cells and measured intracellular iron content, heme-iron uptake and transport and immunolocalization of heme oxygenase-1 in these cells. We found that heme oxygenase-1 expressing cells showed increased apical heme iron uptake and transepithelial transport when compared to control cells. These results suggested that heme oxygenase-1 mediates heme iron influx and efflux in intestinal cells.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Regulación Enzimológica de la Expresión Génica
/
Células Epiteliales
/
Hemo-Oxigenasa 1
/
Hierro
Límite:
Humanos
Idioma:
Inglés
Revista:
Biol. Res
Asunto de la revista:
Biologia
Año:
2006
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Chile
Institución/País de afiliación:
Universidad de Chile/CL
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