Characterization of mitochondrial iron uptake in HepG2 cells
Biol. Res
;
39(1): 199-201, 2006. ilus
Artículo
en Inglés
| LILACS
| ID: lil-430715
RESUMO
There is increasing evidence that accumulation of redox-active iron in mitochondria leads to oxidative damage and contributes to various neurodegenerative diseases, such as Friedreich's ataxia and Parkinson's disease. In this work, we examined the existence of regulatory mechanisms for mitochondrial iron uptake and storage. To that end, we used rhodamine B- [(1,10-phenanthrolin-5-yl)amino carbonyl ] benzyl ester, a new fluorescent iron-sensitive probe that is targeted specifically to the mitochondrion. We found that extracellular iron was incorporated readily into mitochondria in an apparently saturable process. Moreover, the rate of iron incorporation responded to the Fe status of the cell, an indication that the mitochondrion actively regulates its iron content.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Rodaminas
/
Colorantes Fluorescentes
/
Hierro
/
Mitocondrias
Límite:
Humanos
Idioma:
Inglés
Revista:
Biol. Res
Asunto de la revista:
Biologia
Año:
2006
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Chile
/
Israel
Institución/País de afiliación:
Hebrew University of Jerusalem/IL
/
Universidad de Chile/CL
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