The Ca2+ pump inhibitor, thapsigargin, inhibits root gravitropism in Arabidopsis thaliana
Biol. Res
;
39(2): 289-296, 2006. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-432431
RESUMO
Thapsigargin, a specific inhibitor of most animal intracellular SERCA-type Ca2+ pumps present in the sarcoplasmic/endoplasmic reticulum, was originally isolated from the roots of the Mediterranean plant Thapsia gargancia L. Here, we demonstrate that this root-derived compound is capable of altering root gravitropism in Arabidopsis thaliana. Thapsigargin concentrations as low as 0.1 µM alter root gravitropism whereas under similar conditions cyclopiazonic acid does not. Furthermore, a fluorescently conjugated thapsigargin (BODIPY FL thapsigargin) suggests that target sites for thapsigargin are located in intracellular organelles in the root distal elongation zone and the root cap, regions known to regulate root gravitropism.
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Índice:
LILACS (Américas)
Asunto principal:
Arabidopsis
/
ATPasas Transportadoras de Calcio
/
Gravitropismo
/
Tapsigargina
/
Inhibidores Enzimáticos
Idioma:
Inglés
Revista:
Biol. Res
Asunto de la revista:
Biologia
Año:
2006
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Chile
Institución/País de afiliación:
Universidad Andrés Bello/CL
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