Detection of Toxoplasma gondii DNA in sera samples of mice experimentally infected
J. venom. anim. toxins incl. trop. dis
;
12(2): 202-214, 2006. ilus
Artículo
en Inglés
| LILACS
| ID: lil-434718
ABSTRACT
Detection of Toxoplasma gondii (T. gondii) DNA in blood can help to diagnose the disease in its acute phase; however, it must be considered that hemoglobin, present in blood, can inhibit polymerase activity, making impracticable the detection of DNA in samples. Mice were experimentally infected via oral route with ME49 and BTU2 strains cysts and RH strain tachyzoites; polymerase chain reaction was used to detect T. gondii DNA in mice sera 18, 24, 48, 96, and 192 hours post infection (PI). Toxoplama gondii DNA was detected in only one animal infected with BTU2 strain, genotype III (isolated from a dog with neurological signs) 18 hours PI. The agents DNA was not detected in any sample of the other experimental groups. New studies must be carried out to verify the technique sensitivity in researches on this agents genetic material using sera samples of acute-phase toxoplasmosis patients, especially in cases of immunosuppression
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Ratas
/
Pruebas Serológicas
/
Toxoplasmosis
/
Reacción en Cadena de la Polimerasa
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
Idioma:
Inglés
Revista:
J. venom. anim. toxins incl. trop. dis
Asunto de la revista:
Toxicología
Año:
2006
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
São Paulo State University/BR
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