Application of biochemical and polymerase chain reaction assays for identification of Campylobacter isolates from non-human primates
Mem. Inst. Oswaldo Cruz
;
101(5): 499-501, Aug. 2006. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-437033
ABSTRACT
A multiplex polymerase chain reaction (PCR) assay was performed on 167 thermophilic campylobacters isolated from non-human primates. Samples were first identified by phenotypic methods resulting in 64 Campylobacter jejuni and 103 C. coli strains. Four strains identified biochemically as C. coli, were then determined to be C. jejuni by PCR. Comparison of methodologies showed that the main discrepancies were attributed to the hippurate hydrolysis test and sensitivity to cephalothin and nalidixic acid. Analysis of data showed that the application of phenotypic methods should be supplemented by a molecular method to offer a more reliable Campylobacter identification.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Campylobacter
/
ADN Bacteriano
/
Reacción en Cadena de la Polimerasa
/
Técnicas de Tipificación Bacteriana
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2006
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Fundação Oswaldo Cruz/BR
/
Universidade Federal do Rio de Janeiro/BR
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