Schizodeme analysis of Trypanosoma cruzi Colombian strain clones isolated from the acute phase of murine infection
Mem. Inst. Oswaldo Cruz
; 101(6): 613-615, Sept. 2006. ilus
Article
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| LILACS
| ID: lil-437052
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ABSTRACT
Colombian strain of Trypanosoma cruzi, biodeme Type III (T. cruzi I), has been cloned by micromanipulation at two phases of the acute infection early (10 days ) and advanced (30 days). Twelve clones were obtained therefrom. Characterization by their biological and biochemical behavior showed an identity among the several clones and their parental strain, albeit with different degrees of virulence. Molecular characterization of the kinetoplast DNA (kDNA) after amplification by polymerase chain reaction revealed identical profiles of the bands from the kDNA minicircle by the analysis of restriction fragment lenght polymorphism for the isolated clones, their parental strain, and to the clones isolated at two different phases of the infection. Results suggest the predominance of a "principal clone", in the composition of the Colombian strain, responsible for the biological and biochemical behavior. However, no relationship was detected between the molecular profile of kDNA and the degree of virulence presented by the several clones.
Texto completo:
1
Índice:
LILACS
Asunto principal:
Trypanosoma cruzi
/
ADN de Cinetoplasto
Límite:
Animals
País/Región como asunto:
America do sul
/
Colombia
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Año:
2006
Tipo del documento:
Article