Giardia duodenalis: analysis of secreted proteases upon trophozoite-epithelial cell interaction in vitro
Mem. Inst. Oswaldo Cruz
;
101(6): 693-696, Sept. 2006. ilus, tab, graf
Artículo
en Inglés
| LILACS
| ID: lil-437067
ABSTRACT
Protease secretion by Giardia duodenalis trophozoites upon interaction with epithelial cells and its association with the parasite adhesion was studied in co-cultures of parasites with IEC6 epithelial cell monolayers in the presence or absence of protease inhibitors. Proteolytic activity in supernatants from trophozoites was enhanced when they were co-cultured with IEC6 cells. This activity was strongly inhibited by pre-incubation of live trophozoites with E-64 and TPCK and a concomitant inhibition of parasite adhesion to IEC6 cells was observed. These data suggest that trophozoites secrete cysteine-type proteases that play a role in the adhesion of G. duodenalis to epithelial cells.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Péptido Hidrolasas
/
Inhibidores de Proteasas
/
Células Epiteliales
/
Giardia
Límite:
Animales
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2006
Tipo del documento:
Artículo
País de afiliación:
México
Institución/País de afiliación:
Centro Médico Nacional Siglo XXI/MX
/
Instituto Mexicano del Seguro Social/MX
/
Universidad Autónoma de Yucatán/MX
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