Histidines 13 and 14 in the Aâ sequence are targets for inhibition of Alzheimer's disease Aâ ion channel and cytotoxicity
Biol. Res
;
39(3): 447-460, 2006. ilus
Artículo
en Inglés
| LILACS
| ID: lil-437378
ABSTRACT
The fact that Alzheimer's beta amyloid (Aâ) peptides forms cation channels in lipid bilayers was discovered during the course of our experiments in the laboratory of "Guayo" Rojas at NIH in Bethesda, Maryland (USA). Recently, we found that the Aâ ion channel could be blocked selectively with small peptides that copy the amino acid sequence of the predicted mouth region of the Aâ channel pore. We now have searched for the essential amino acid residues required for this blocking effect by mutations. We found that the ability of peptides to block Aâ channel activity could be lost by replacement of histidines 13 and 14 by alanine or lysine. The amino acid substitution also resulted in the loss of the capacity of the peptides to protect cells from Aâ cytotoxicity. These data thus contribute to the definition of the region of the Aâ sequence that participates in the formation of the channel pore. Additionally, these data support the hypothesis that the ion channel activity of Ab contributes significantly to the cytotoxic properties of Aâ. These data also emphasize the potential value in using inhibition of Aâ ion channel activity as an end point for Alzheimer's disease drug discovery.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Péptidos beta-Amiloides
/
Enfermedad de Alzheimer
/
Histidina
/
Canales Iónicos
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Biol. Res
Asunto de la revista:
Biologia
Año:
2006
Tipo del documento:
Artículo
País de afiliación:
Estados Unidos
Institución/País de afiliación:
Uniformed Services University/US
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