Protein kinase C isoform specificity of cholinergic potentiation of glucose-induced pulsatile 5-HT/ insulin release from mouse pancreatic islets
Biol. Res
;
39(3): 531-539, 2006. ilus
Artículo
en Inglés
| LILACS
| ID: lil-437385
ABSTRACT
Thymeleatoxin (TMX), an activator of Ca2+-sensitive protein kinase C (cPKC) isoforms, was used to assess the PKC isoform specificity of cholinergic potentiation of glucose (11 mM)-induced pulsatile 5-HT/insulin release (PIR) from single mouse pancreatic islets. TMX (100 nM) and carbachol (Cch, 50 mM) enhanced PIR ~ 3-fold while reducing the underlying [Ca2+]i oscillations (duration and amplitude) by ~ 40-50 percent. Both effects were ablated by the specific PKC inhibitor bisindolylmaleimide and chronic TMX pretreatment. Cch also evoked an initial transient [Ca2+]i rise and surge of 5-HT release, which remained unaffected by chronic TMX pretreatment. It is concluded that the immediate cholinergic responses are insensitive to cPKC. In contrast, specific activation of a cPKC isoform mediates sustained cholinergic potentiation of glucose-induced insulin secretion.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Proteína Quinasa C
/
Ésteres del Forbol
/
Serotonina
/
Islotes Pancreáticos
/
Glucosa
/
Insulina
Límite:
Animales
Idioma:
Inglés
Revista:
Biol. Res
Asunto de la revista:
Biologia
Año:
2006
Tipo del documento:
Artículo
País de afiliación:
Portugal
Institución/País de afiliación:
University of Coimbra/PT
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