Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil
Mem. Inst. Oswaldo Cruz
;
101(7): 721-724, Nov. 2006. tab
Artículo
en Inglés
| LILACS
| ID: lil-439454
ABSTRACT
The objective of this study was to describe the circulation of caliciviruses in the West Central region of Brazil and its correlation with children's gender and age, as well as with the year and months of the sample collection. Reverse transcriptase-polymerase chain reaction was performed to detect the human calicivirus genome in 1006 fecal samples that were collected in Goiânia (n = 696) and Brasília (n = 310). Viral RNA was detected in 8.6 percent of the samples. No significant difference in viral prevalence was found regarding gender, age or year of the sample. However, it was observed that in Goiânia, there is a higher incidence of caliciviruses from September to March. The analysis employing three primer pairs demonstrated that the Ni/E3 or JV12/13 primer pairs, which detect norovirus (NoV), detected 41 positive samples while the 289/290 primer pair, which detects NoV or sapovirus, detected the remaining 46 samples. Calicivirus circulates in the West Central region of Brazil and for better detection of this virus it is important to use more than one primer pair. Also, we conclude that the seasonality presented by this virus is related to higher humidity in the period.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Caliciviridae
/
Infecciones por Caliciviridae
/
Heces
/
Gastroenteritis
Tipo de estudio:
Estudio diagnóstico
/
Estudio de prevalencia
/
Factores de riesgo
Límite:
Child, preschool
/
Humanos
/
Lactante
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2006
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Instituto Oswaldo Cruz/BR
/
Instituto de Patologia Tropical e Saúde Pública/BR
/
Instituto de Saúde do Distrito Federal/BR
/
Universidade Federal de Goiás/BR
/
Universidade de Brasília/BR
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