Detection of genetic polymorphism among and within Echinococcus granulosus strains by heteroduplex analysis of a microsatellite from the U1 snRNA genes
Genet. mol. res. (Online)
;
5(3): 542-552, 2006. ilus, tab, graf
Artículo
en Inglés
| LILACS
| ID: lil-441050
ABSTRACT
Polymerase chain reaction of a pentanucleotide microsatellite in the U1 snRNA gene complex generated a multiple band pattern due to the priming of paralogous sequences. Denaturation and slow renaturation of polymerase chain reaction products allow the formation of heteroduplex DNA that can be detected by its differential mobility in polyacrylamide gel electrophoresis. Heteroduplex analysis was used to determine if the U1 snRNA microsatellite could be a useful genetic marker in Echinococcus granulosus. A U1 snRNA microsatellite fragment from E. granulosus was isolated and characterized by Southern blot and sequencing. Four E. granulosus strains were analyzed sheep, Tasmanian sheep, cattle, and camel strains. The former two showed polymorphism and shared three of the six patterns found for sheep strain. The cattle strain displayed two patterns, and the camel strain was monomorphic. The electrophoretic profiles were used for statistical analysis in order to determine genetic distance and the relationship among strains. Heteroduplex analysis can be helpful in genotyping E. granulosus strains and is useful in detecting polymorphism within strains.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Polimorfismo Genético
/
Ribonucleoproteína Nuclear Pequeña U1
/
Repeticiones de Microsatélite
/
Echinococcus granulosus
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Genet. mol. res. (Online)
Asunto de la revista:
Biologia Molecular
/
Genética
Año:
2006
Tipo del documento:
Artículo
País de afiliación:
Argentina
/
Brasil
Institución/País de afiliación:
ANLIS Dr. Carlos G. Malbrán/AR
/
Universidade Federal de Santa Maria/BR
/
Universidade Federal do Rio Grande do Sul/BR
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