Application of synthetic peptides in development of a serologic method for laboratory diagnosis of schistosomiasis mansoni
Mem. Inst. Oswaldo Cruz
;
101(supl.1): 355-357, Oct. 2006. tab, graf
Artículo
en Inglés
| LILACS
| ID: lil-441274
ABSTRACT
The immunoreactivity of seven peptides synthesized from Schistosoma mansoni proteins, was evaluated by dot-blot and ELISA assays using two different sensitization methodologies. The best results were obtained on wells of the Costar 3590 microplates coated with peptides P1, P2, P3, P6, and P7 using conventional methodology. The signals increased considerably (p < 0.0003) on wells sensitized with P1 to P6 using alternative methodology. In contrast, the well coated with peptide P7 presented lower signal when compared with conventional methodology (p = 0.0019). These results, establish the basis for the application of synthetic peptides for laboratory diagnosis of schistosomiasis mansoni.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Péptidos
/
Esquistosomiasis mansoni
/
Proteínas del Helminto
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2006
Tipo del documento:
Artículo
País de afiliación:
Brasil
/
Estados Unidos
Institución/País de afiliación:
Center for Biologics Evaluation and Research/US
/
Universidade de São Paulo/BR
Similares
MEDLINE
...
LILACS
LIS