Analysis of the population structure of Uruguayan Creole cattle as inferred from milk major gene polymorphisms
Genet. mol. biol
;
29(3): 491-495, 2006. tab
Artículo
en Inglés
| LILACS
| ID: lil-450287
ABSTRACT
The ancestors of Uruguayan Creole cattle were introduced by the Spanish conquerors in the XVII century, following which the population grew extensively and became semi-feral before the introduction of selected breeds. Today the Uruguayan Creole cattle genetic reserve consists of 575 animals. We used the tetra primer amplification refractory mutation system polymerase chain reaction (ARMS-PCR) to analyze the kappa-casein, beta-casein, alphaS1-casein and alpha-lactoalbumin gene polymorphisms and restriction fragment length polymorphism PCR (RFLP-PCR) for the beta-lactoglobulin and the acylCoAdiacyl glycerol acyltransferase 1 (DGAT1) genes. The kappa-casein and beta-lactoglobulin genes presented very similar A and B allele frequencies, while the alphas1-casein and alpha-lactoalbumin gene B alleles showed much higher frequencies than the corresponding A alleles. The beta-casein B allele was not found in the population sampled. There was a very high frequency of the DGAT1 gene A allele which is associated with low milk fat content and high milk yield. All loci were in Hardy-Weinberg equilibrium and the level of heterozygosity agreed with the high genetic diversity observed in a previous analysis of this population. Preservation of the allelic richness observed in the Uruguayan Creole cattle should be considered for future dairy management and livestock genetic improvement. The results also emphasize the value of the tetra primers ARMS-PCR technique as a rapid, easy and economical way of genotyping cattle breeds for milk gene single nucleotide polymorphisms.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Bovinos
/
Polimorfismo de Nucleótido Simple
/
Diacilglicerol O-Acetiltransferasa
/
Proteínas de la Leche
Límite:
Animales
País/Región como asunto:
America del Sur
/
Uruguay
Idioma:
Inglés
Revista:
Genet. mol. biol
Asunto de la revista:
Genética
Año:
2006
Tipo del documento:
Artículo
País de afiliación:
Uruguay
Institución/País de afiliación:
Facultad de Veterinaria/UY
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