Isolation and molecular identification of Leishmania ( Viannia ) peruviana from naturally infected Lutzomyia peruensis (Diptera: Psychodidae) in the Peruvian Andes
Mem. Inst. Oswaldo Cruz
;
102(5): 655-658, Aug. 2007. ilus
Artículo
en Inglés
| LILACS
| ID: lil-458635
ABSTRACT
Leishmania (Viannia) peruviana was isolated from 1/75 Lutzomyia peruensis captured during May 2006 in an endemic cutaneous leishmaniasis region of the Peruvian Andes (Chaute, Huarochiri, Lima, Peru). Sand fly gut with promastigotes was inoculated into a hamster and the remaining body was fixed in ethanol. L. (Viannia) sp. was determined by polymerase chain reaction (PCR), and Leishmania species through molecular genotyping by PCR-restriction fragment length polymorphism analyses targeting the genes cpb and hsp70, resulting L. (V.) peruviana. The infected sand fly appeared 15 days after the rains finished, time expected and useful real time data for interventions when transmission is occurring.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Psychodidae
/
Leishmania braziliensis
/
ADN Protozoario
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
País/Región como asunto:
America del Sur
/
Perú
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2007
Tipo del documento:
Artículo
País de afiliación:
Perú
Institución/País de afiliación:
Universidad Peruana Cayetano Heredia/PE
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