Polymorphism of exon 2-3 of bovine major histocompatibility complex class I BoLa-A gene
Genet. mol. biol
;
30(3): 560-566, 2007. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-460070
ABSTRACT
The exon 2-3 region of bovine major histocompatibility complex (MHC) class I BoLa-A gene was investigated for polymorphisms in three breeds of cattle originated in the Indian subcontinent namely Sahiwal, Tharparkar, Hariana, as well as crossbred (Bos taurus x Bos indicus) cattle and Jersey, the exotic breed (Bos taurus). The PCR amplified fragment of 714 bp showed distinct DdeI-, TaqI- and HinfI- RFLP patterns, thus confirming a higher degree of polymorphism in this region. To our knowledge this is the first report of HinfI restriction patterns for BoLa-A exon 2-3. The sequencing results revealed a number of nucleotide substitutions in this region, which resulted in amino acid changes. The present investigation confirmed that MHC class I BoLa-A exon 2-3 is highly polymorphic in cattle.
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Índice:
LILACS (Américas)
Idioma:
Inglés
Revista:
Genet. mol. biol
Asunto de la revista:
Genética
Año:
2007
Tipo del documento:
Artículo
País de afiliación:
India
Institución/País de afiliación:
Indian Veterinary Research Institute/IN
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