The chromatin structure of the lysozyme GAS41 origin of DNA replication changes during the cell cycle
Biol. Res
;
40(2): 185-192, 2007. ilus
Artículo
en Inglés
| LILACS
| ID: lil-468189
ABSTRACT
We used a rapid and simple protocol using lysolecithin for mapping HS sites in vivo. The protocol is based on partial digestion with DNase I of exponentially growing cells following permeabilization by short treatment with lysolecithin. Using this protocol, we analyzed the chromatin structure of the region surrounding two overlapping elements, an origin of bidirectional DNA replication and the GAS41 promoter, in chicken myelomonocytic HD11 cells arrested in G0, G0 and S phases as well as at the G0/S border. The results show that the chromatin of this region became more nuclease sensitive when cells were arrested in G0 phase and that this change in chromatin structure was reversible after the cells began to enter S phase.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Factores de Transcripción
/
Cromatina
/
Ciclo Celular
/
Replicación del ADN
Tipo de estudio:
Guía de Práctica Clínica
Límite:
Humanos
Idioma:
Inglés
Revista:
Biol. Res
Asunto de la revista:
Biologia
Año:
2007
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Alemania
Institución/País de afiliación:
Federal Agricultural Research Centre/DE
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