Construction of a recombinant plasmid pSH-G containing the rabies-virus glycoprotein G gene
J. venom. anim. toxins incl. trop. dis
;
13(4): 874-880, 2007. ilus
Artículo
en Inglés
| LILACS
| ID: lil-471147
ABSTRACT
A plasmid named pSH-G was constructed with the rabies-virus G-gene insert. This plasmid was transfected into eukaryotic BHK-21 cells and its stability tested. The presence of the pSH-G plasmid was confirmed by means of polymerase chain reaction (PCR) after each of ten cell passages, and the results were positive. The stable BHK-21/pSH-G+ clone obtained can be used in the study of rabies as well as in the production of vaccines.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Plásmidos
/
Virus de la Rabia
/
Proteínas Recombinantes de Fusión
/
Glicoproteínas
/
Transfección
Idioma:
Inglés
Revista:
J. venom. anim. toxins incl. trop. dis
Asunto de la revista:
Toxicología
Año:
2007
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Pasteur Institute/BR
/
University of São Paulo/BR
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