ELISA indireto para detecção de IgG antivírus da doença de Newcastle em soro de codorna / Indirect ELISA for the detection of IgG specific to Newcastle disease virus in quail serum
Arq. bras. med. vet. zootec
;
59(5): 1344-1347, out. 2007. ilus, tab
Artículo
en Portugués
| LILACS
| ID: lil-471226
ABSTRACT
An indirect ELISA for the detection of japanese quail IgG specific to Newcastle disease virus (NDV) was developed. The secondary anti-quail IgG was produced in Balb/c mice, by inoculating Freund's complete adjuvant emulsified japanese quail-IgG extract. The purification of IgG was achieved using the caprilic acid method. The ELISA was compared to the haemagglutination-inhibition (HI) test for antibodies to NDV. ELISA cut-off point was established through TG-ROC analysis. Total correlation was observed between the ELISA and the HI, being the ELISA efficient in the identification of positive and negative sera, with high sensitivity and specificity (100 percent). These results validate the use of the indirect ELISA as an alternative for the detection of NDV-specific IgG in japanese quail sera, with the advantage of high sensitivity and automation
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Virus de la Enfermedad de Newcastle
/
Inmunoglobulina G
/
Ensayo de Inmunoadsorción Enzimática
/
Coturnix
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
Idioma:
Portugués
Revista:
Arq. bras. med. vet. zootec
Asunto de la revista:
Medicina Veterinaria
Año:
2007
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
PUC-Minas/BR
/
UFMG/BR
/
UNIPAC/BR
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