Evaluation of a modified culture medium for Borrelia burgdorferi sensu lato
Mem. Inst. Oswaldo Cruz
;
102(8): 999-1002, Dec. 2007. ilus
Artículo
en Inglés
| LILACS
| ID: lil-471853
ABSTRACT
The aim of the present study was to assess the possible use of a modified medium, prepared in the laboratory using the constituents of Barbour-Stonner-Kelly (BSK) medium and medium 199 as base, for the culture of Borrelia strains, comparing the growth of individual strains in this medium and in the BSK-H medium, and the protein profile and antigenic characteristics of Borrelia proteins expressed in these media. A qualitative evaluation of growth of Borrelia species was made with acceptable results (morphology and motility), but during a quantitative evaluation using the three main genospecies of Borrelia, the better results were obtained with a B. burgdorferi sensu stricto strain. The modified medium did not enable the growth of a B. afzelii strain. The protein profile and antigenic characteristic of the expressed proteins in the modified medium were studied with satisfactory results. These results suggest the modified medium as an alternative for the cultivation of Borrelia strains, with some limitations, in poorly-resourced laboratories.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Grupo Borrelia Burgdorferi
/
Medios de Cultivo
Tipo de estudio:
Estudios de evaluación
/
Investigación cualitativa
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2007
Tipo del documento:
Artículo
País de afiliación:
Cuba
/
Suiza
Institución/País de afiliación:
ADMed/CH
/
Tropical Medicine Institute Pedro Kourí/CU
/
University of Neuchâtel/CH
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