DNA testing for parentage verification in a conservation nucleus of Pantaneiro horse
Genet. mol. biol
;
31(1): 64-67, 2008. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-476153
ABSTRACT
We investigated the genealogy of the in situ conservation nucleus of the Pantaneiro horse using DNA microsatellites by evaluating 101 horses, the group consisting of 71 adult horses (3 stallions, 40 male and 31 mares) and 27 foals (14 colts and 13 fillies). Genomic DNA was extracted from hair roots and genotyped using 12 microsatellite markers (AHT4, AHT5, ASB2, ASB17, ASB23, HMS3 HMS6, HMS7, HTG4, HTG10, LEX33 and VHL20). The number of alleles per locus varied from 6 to 13, with a mean of 7.8 and the expected heterozygosity ranged from 0.544 to 0.734 (mean 0.644). The VLH20, ASB2, HTG10, ASB23 markers had a high (> 0.8) polymorphism information content and the total exclusion probability of the 12 microsatellite loci was 0.99. The genealogical study of the Pantaneiro horse using genetic markers was efficient in detecting mistakes during paternity and maternity designation and is an important tool which can be used together with traditional systems of animal identification. The use of genetic markers is recommended in the systematic control of the genealogical registrations and conservation plans to improve genetic aspects of the Pantaneiro horse.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
ADN
/
Repeticiones de Microsatélite
/
Caballos
Límite:
Animales
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Genet. mol. biol
Asunto de la revista:
Genética
Año:
2008
Tipo del documento:
Artículo
País de afiliación:
Brasil
/
España
Institución/País de afiliación:
Embrapa Cerrados/BR
/
Laboratorio de Genética Molecular/ES
/
Universidad de Córdoba/ES
/
Universidade de Brasília/BR
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