Adenoviruses C in non-hospitalized Mexican children older than five years of age with acute respiratory infection
Mem. Inst. Oswaldo Cruz
;
103(2): 195-200, Mar. 2008. graf, tab
Artículo
en Inglés
| LILACS
| ID: lil-480634
ABSTRACT
Adenoviruses (AdV) are commonly involved in acute respiratory infections (ARI), which cause high morbidity and mortality in children. AdV are grouped in six species (A-F), which are associated with a wide range of diseases. The aim of this study was to identify the AdV species infecting non-hospitalized Mexican children with ARI symptoms, attending to the same school. For that, a PCR/RFLP assay was designed for a region of the hexon gene, which was chosen, based on the bioinformatical analysis of AdV genomes obtained from GenBank. A total of 100 children's nasopharyngeal samples were collected from January to June, 2005, and used for viral isolation in A549 cells and PCR/RFLP analysis. Only 15 samples produced cytopathic effect, and in all of them AdV C was identified. AdV C was also identified in eight additional nasopharyngeal samples which were negative for viral isolation. In summary, this outpatient population showed a rate of AdV infection of 23 percent, and only AdV C was detected.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Infecciones del Sistema Respiratorio
/
Infecciones por Adenovirus Humanos
/
Adenovirus Humanos
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
Límite:
Niño
/
Femenino
/
Humanos
/
Masculino
País/Región como asunto:
México
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2008
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
México
Institución/País de afiliación:
Instituto Nacional de Enfermedades Respiratorias/MX
/
Instituto Politécnico Naciona/MX
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