Analysis of blood processing conditions to obtain high-quality total RNA from human leukocyte concentrate
Genet. mol. res. (Online)
;
6(2): 298-307, 2007. tab, graf
Artículo
en Inglés
| LILACS
| ID: lil-482040
ABSTRACT
Blood samples are used as a biological source to discover biomarkers of hematological and non-hematological disorders. The present study shows the impact of different experimental conditions associated with cell lysis buffer, TRI-reagent protocol and blood cell storage buffer and their correlation with the quantity, quality and Adrenomedullin gene expression levels of total RNA when RT-PCR technique is used. A leukocyte cell bank protocol is also proposed for further mRNA expression analysis using RNAlater as storage buffer. There is evidence that total RNA isolated from leukocyte concentrate stored for 1 month at -70 degrees C did not show significant differences concerning quality, purity and Adrenomedullin gene expression compared with the freshly processed leukocyte sample.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Conservación de la Sangre
/
ARN
/
ARN Mensajero
/
Leucocitos
Límite:
Humanos
Idioma:
Inglés
Revista:
Genet. mol. res. (Online)
Asunto de la revista:
Biologia Molecular
/
Genética
Año:
2007
Tipo del documento:
Artículo
País de afiliación:
Cuba
Institución/País de afiliación:
Center for Genetic Engineering and Biotechnology/CU
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