Sperm defect severity rather than sperm source is associated with lower fertilization rates after intracytoplasmic sperm injection
Int. braz. j. urol
;
34(1): 49-56, Jan.-Feb. 2008. tab
Artículo
en Inglés
| LILACS
| ID: lil-482942
ABSTRACT
OBJECTIVE:
To evaluate the impact of sperm defect severity and the type of azoospermia on the outcomes of intracytoplasmic sperm injection (ICSI). MATERIALS ANDMETHODS:
This study included 313 ICSI cycles that were divided into two major groups according to the source of spermatozoa used for ICSI 1) Ejaculated (group 1; n = 220) and 2) Testicular/Epididymal (group 2; n = 93). Group 1 was subdivided into four subgroups according to the results of the semenanalysis:
1) single defect (oligo-[O] or astheno-[A] or teratozoospermia-[T], n = 41), 2) double defect (a combination of two single defects, n = 45), 3) triple defect (OAT, n = 48), and 4) control (no sperm defects; n = 86). Group 2 was subdivided according to the type of azoospermia 1) obstructive (OA n = 39) and 2) non-obstructive (NOA n = 54). Fertilization (2PN), cleavage, embryo quality, clinical pregnancy and miscarriage rates were statistically compared using one-way ANOVA and Chi-square analyses.RESULTS:
Significantly lower fertilization rates were obtained when either ejaculated sperm with triple defect or testicular sperm from NOA patients (63.4 + 25.9 percent and 52.2 + 29.3 percent, respectively) were used for ICSI as compared to other groups (~73 percent; P < 0.05). Epididymal and testicular spermatozoa from OA patients fertilized as well as normal or mild/moderate deficient ejaculated sperm. Cleavage, embryo quality, pregnancy and miscarriage rates did not differ statistically between ejaculated and obstructive azoospermia groups. However, fertilization, cleavage and pregnancy rates were significantly lower for NOA patients.CONCLUSION:
Lower fertilization rates are achieved when ICSI is performed with sperm from men with oligoasthenoteratozoospermic and non-obstructive azoospermic, and embryo development and pregnancy rates are significantly lower when testicular spermatozoa from NOA men are used.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Espermatozoides
/
Inyecciones de Esperma Intracitoplasmáticas
/
Recuperación de la Esperma
/
Fertilización
Tipo de estudio:
Estudio observacional
/
Factores de riesgo
Límite:
Adulto
/
Femenino
/
Humanos
/
Masculino
/
Embarazo
Idioma:
Inglés
Revista:
Int. braz. j. urol
Asunto de la revista:
Urología
Año:
2008
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Androfert, Center for Male Reproduction/BR
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