Hepatitis A virus subgenotypes dissemination during a community outbreak in a surrounding region of Rio de Janeiro
Mem. Inst. Oswaldo Cruz
;
103(3): 254-258, May 2008. ilus, graf, mapas, tab
Artículo
en Inglés
| LILACS
| ID: lil-485214
ABSTRACT
From December 1999 to December 2001, many cases of hepatitis A were notified in the county of Belford Roxo involving individuals aged 0 to 79 years. Serum samples were collected to evaluate the prevalence of anti-hepatitis A virus (HAV) antibodies, to detect HAV-RNA and to correlate with possible risk factors of HAV infection. Serum samples were screened by commercial IgM and total anti-HAV antibody ELISA and HAV-RNA was isolated and subsequently amplified by reverse transcription-polymerase chain reaction (RT-PCR) at VP1/2A region, sequenced and analyzed. Total anti-HAV prevalence was 87.9 percent (203/231) and IgM anti-HAV prevalence was 38.7 percent (89/231). Multivariate analysis showed that individuals under 20 years old are risks groups to acquire the infection suggesting that hygienic habits of young subjects are the principal factor of transmission and so they could be the target for vaccine programs. HAV-RNA was amplified from 29 (32.5 percent) IgM anti-HAV positive patients and 26 samples were sequenced and classified into subgenotypes IB (8 isolates) and IA (18 isolates). Isolates classified into subgenotype IB were identical representing one distinct strain. We could observe both subgenotypes circulating during the study which suggests different sources of infection. Prophylactic measures as vaccination strategies added to improvements in hygienic and sanitary conditions would be highly effective to reduction of infection.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Inmunoglobulina M
/
ARN Viral
/
Brotes de Enfermedades
/
Virus de la Hepatitis A Humana
/
Anticuerpos de Hepatitis A
/
Hepatitis A
Tipo de estudio:
Estudio de etiología
/
Estudio de prevalencia
/
Factores de riesgo
Límite:
Adolescente
/
Adulto
/
Anciano
/
Niño
/
Child, preschool
/
Femenino
/
Humanos
/
Lactante
/
Masculino
/
Recién Nacido
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2008
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Fiocruz/BR
Similares
MEDLINE
...
LILACS
LIS