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Polymerase chain reaction genotyping of Epstein-Barr virus in scraping samples of the tongue lateral border in HIV-1 seropositive patients
Robaina, T. F; Valladares, C. P; Tavares, D. S; Napolitano, W. C; Silva, L. E; Dias, E. P; Leite, J. P. G.
  • Robaina, T. F; s.af
  • Valladares, C. P; s.af
  • Tavares, D. S; s.af
  • Napolitano, W. C; s.af
  • Silva, L. E; Universidade Federal Fluminense. Instituto de Matemática. Niterói. BR
  • Dias, E. P; Universidade Federal Fluminense. Programa de Pós-graduação em Patologia. Niterói. BR
  • Leite, J. P. G; Fiocruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada. Rio de Janeiro. BR
Mem. Inst. Oswaldo Cruz ; 103(4): 326-331, June 2008. ilus, graf, tab
Artículo en Inglés | LILACS | ID: lil-486872
ABSTRACT
The Epstein-Barr virus (EBV) is the etiological agent of oral hairy leukoplakia (OHL), an oral lesion with important diagnostic and prognostic value in acquired immunodeficiency disease syndrome. The two EBV genotypes, EBV-1 and EBV-2, can be distinguished by divergent gene sequences encoding the EBNA-2, 3A, 3B, and 3C proteins. The purpose of this study was to identify the EBV genotype prevalent in 53 samples of scrapings from the lateral border of the tongue of HIV-1 seropositive patients, with and without OHL, and to correlate the genotypes with presence of clinical or subclinical OHL with the clinic data collected. EBV-1 and EBV-2 were identified through PCR and Nested-PCR based on sequence differences of the EBNA-2 gene. EBV-1 was identified in the 31 samples (15 without OHL, 7 with clinical OHL and 9 with subclinical OHL), EBV-2 in 12 samples (10 without OHL, 1 with clinical and 1 subclinical OHL), and a mixed infection in 10 samples (2 without OHL, 3 with clinical and 5 with subclinical OHL). The presence of EBV-1 was higher in women, but a significant statistical result relating one the EBV genotypes to the development of OHL was not found. We conclude that the oral epithelium in HIV-1 seropositive patients can be infected by EBV-1, EBV-2 or by a mixed viral population.
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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Lengua / VIH-1 / Infecciones Oportunistas Relacionadas con el SIDA / Leucoplasia Vellosa Límite: Adulto / Anciano / Femenino / Humanos / Masculino Idioma: Inglés Revista: Mem. Inst. Oswaldo Cruz Asunto de la revista: Medicina Tropical / Parasitología Año: 2008 Tipo del documento: Artículo País de afiliación: Brasil Institución/País de afiliación: Fiocruz/BR / Universidade Federal Fluminense/BR

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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Lengua / VIH-1 / Infecciones Oportunistas Relacionadas con el SIDA / Leucoplasia Vellosa Límite: Adulto / Anciano / Femenino / Humanos / Masculino Idioma: Inglés Revista: Mem. Inst. Oswaldo Cruz Asunto de la revista: Medicina Tropical / Parasitología Año: 2008 Tipo del documento: Artículo País de afiliación: Brasil Institución/País de afiliación: Fiocruz/BR / Universidade Federal Fluminense/BR