Identification and detection of methicillin resistance in Non-Epidermidis coagulase-negative staphylococci
Braz. j. infect. dis
;
12(4): 316-320, Aug. 2008. tab
Artículo
en Inglés
| LILACS
| ID: lil-496771
ABSTRACT
The NCCLS (2004) presented a new methodology to detect, by disk-diffusion agar, oxacillin-resistance using a cefoxitin disk. We identified coagulase-negative staphylococci (SCoN) to the species level and compared the use of cefoxitin disks (30 µg) with oxacillin disks (1 µg), agar dilution (minimum inhibitory concentration of oxacillin) and mecA gene detection in isolates of coagulase-negative bacteria other than Staphylococcus epidermidis (SCoNne). A total of 238 SCoNne was evaluated; oxacillin-resistance (the mecA gene) was detected in 71 percent of the isolates. All methods gave 100 percent sensitivity, based on presence of the mecA gene. The specificity of the cefoxitin disk was 100 percent, while the oxacillin disk gave a specificity of 91 percent and agar dilution oxacillin gave a specificity of 88 percent. We conclude that the cefoxitin disk is an efficient test, and it is an easy method for use in clinical laboratories to detect oxacillin-resistance in staphylococci.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Staphylococcus
/
Proteínas Bacterianas
/
Resistencia a la Meticilina
/
Pruebas Antimicrobianas de Difusión por Disco
/
Antibacterianos
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
Límite:
Humanos
Idioma:
Inglés
Revista:
Braz. j. infect. dis
Asunto de la revista:
Enfermedades Transmisibles
Año:
2008
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Federal University of Health Sciences of Porto Alegre/BR
/
Weinmann Laboratory/BR
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