Characterization of Shigella spp. by antimicrobial resistance and PCR detection of ipa genes in an infantile population from Porto Velho (Western Amazon region), Brazil
Mem. Inst. Oswaldo Cruz
;
103(7): 731-733, Nov. 2008. tab
Artículo
en Inglés
| LILACS
| ID: lil-498384
ABSTRACT
The incidence of Shigella spp. was assessed in 877 infants from the public hospital in Rondônia (Western Amazon region, Brazil) where Shigella represents the fourth cause of diarrhea. Twenty-five isolates were identified 18 were Shigella flexneri, three Shigella sonnei, three Shigella boydii and one Shigella dysenteriae. With the exception of S. dysenteriae, all Shigella spp. isolated from children with diarrhea acquired multiple antibiotic resistances. PCR detection of ipa virulence genes and invasion assays of bloody diarrhea and fever (colitis) were compared among 25 patients testing positive for Shigella. The ipaH and ipaBCD genes were detected in almost all isolates and, unsurprisingly, all Shigella isolates associated with colitis were able to invade HeLa cells. This work alerts for multiple antibiotic resistant Shigella in the region and characterizes presence of ipa virulence genes and invasion phenotypesin dysenteric shigellosis.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Shigella
/
Colitis
/
Diarrea
/
Disentería Bacilar
Tipo de estudio:
Estudio diagnóstico
/
Estudio de incidencia
/
Estudio pronóstico
Límite:
Child, preschool
/
Humanos
/
Lactante
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2008
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Centro de Pesquisa em Medicina Tropical/BR
/
Fiocruz/BR
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