Un método de transformación genética de maíz para conferirle resistencia ulterior a enfermedades virales / A method for genetic transformation of maize for resistance to viral diseases
Rev. biol. trop
;
52(3): 787-793, sept. 2004. ilus, tab
Artículo
en Español
| LILACS
| ID: lil-501703
ABSTRACT
A system for the genetic transformation of maize was developed for two Costa Rican varieties CR-7 and Diamantes 8843, that can allow the subsequent transfer of viral-derived genes in order to confer resistance to the disease caused by maize rayado fino virus (MRFV). The method is based on particle bombardment of organogenic calli derived from shoot tips. On the other hand, the molecular construction pRFcp-bar, containing the coat protein gene of MRFV and the marker gene bar, was elaborated. For the visual selection of the transformed material was used also the plasmid pDM803 that contains the reporter gene uidA (GUS). The results indicate that devices evaluated the PIG ("Particle Inflow Gun") and the Bio-Rad are both enough efficient to transfer foreign genes to the genome of the maize.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Transformación Genética
/
Plantas Modificadas Genéticamente
/
Zea mays
País/Región como asunto:
America Central
/
Costa Rica
Idioma:
Español
Revista:
Rev. biol. trop
Asunto de la revista:
Biologia
/
Medicina Tropical
Año:
2004
Tipo del documento:
Artículo
País de afiliación:
Costa Rica
Institución/País de afiliación:
Universidad de Costa Rica/CR
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