A model system to study antimicrobial strategies in endodontic biofilms
J. appl. oral sci
;
17(2): 87-91, Mar.-Apr. 2009. ilus
Artículo
en Inglés
| LILACS
| ID: lil-503984
ABSTRACT
The purpose of this work was to develop a model system to study antimicrobial strategies in endodontic biofilms. Enterococcus faecalis suspension was colonized in 10 human root canals. Five milliliters of Brain Heart Infusion (BHI) were mixed with 5 mL of the bacterial inoculums (E. faecalis) and inoculated with sufficient volume to fill the root canal during 60 days. This procedure was repeated every 72 h, always using 24-h pure culture prepared and adjusted to No. 1 MacFarland turbidity standard. Biofilm formation was analyzed by scanning electron microscopy (SEM). E. faecalis consistently adhered to collagen structure, colonized dentin surface, progressed towards the dentinal tubules and formed a biofilm. The proposed biofilm model seems to be viable for studies on antimicrobial strategies, and allows for a satisfactory colonization time of selected bacterial species with virulence and adherence properties.
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Índice:
LILACS (Américas)
Asunto principal:
Enterococcus faecalis
/
Biopelículas
/
Cavidad Pulpar
/
Modelos Biológicos
Límite:
Humanos
Idioma:
Inglés
Revista:
J. appl. oral sci
Asunto de la revista:
Odontología
Año:
2009
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Federal University of Goiás/BR
/
Federal University of Paraná/BR
/
Pontifical Catholic University of Rio Grande do Sul/BR
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