Hidden heterochromatin: characterization in the Rodentia species Cricetus cricetus, Peromyscus eremicus (Cricetidae) and Praomys tullbergi (Muridae)
Genet. mol. biol
;
32(1): 56-68, 2009. ilus
Artículo
en Inglés
| LILACS
| ID: lil-505781
ABSTRACT
The use of in situ restriction endonuclease (RE) (which cleaves DNA at specific sequences) digestion has proven to be a useful technique in improving the dissection of constitutive heterochromatin (CH), and in the understanding of the CH evolution in different genomes. In the present work we describe in detail the CH of the three Rodentia species, Cricetus cricetus, Peromyscus eremicus (family Cricetidae) and Praomys tullbergi (family Muridae) using a panel of seven REs followed by C-banding. Comparison of the amount, distribution and molecular nature of C-positive heterochromatin revealed molecular heterogeneity in the heterochromatin of the three species. The large number of subclasses of CH identified in Praomys tullbergi chromosomes indicated that the karyotype of this species is the more derived when compared with the other two genomes analyzed, probably originated by a great number of complex chromosomal rearrangements. The high level of sequence heterogeneity identified in the CH of the three genomes suggests the coexistence of different satellite DNA families, or variants of these families in these genomes.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Heterocromatina
/
Cricetinae
/
Peromyscus
/
Muridae
Límite:
Animales
Idioma:
Inglés
Revista:
Genet. mol. biol
Asunto de la revista:
Genética
Año:
2009
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Portugal
Institución/País de afiliación:
University of Trás-os-Montes and Alto Douro/PT
Similares
MEDLINE
...
LILACS
LIS