Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples
Mem. Inst. Oswaldo Cruz
;
104(1): 118-120, Feb. 2009. ilus
Artículo
en Inglés
| LILACS
| ID: lil-507216
ABSTRACT
Human adenovirus (HAdV) and human respiratory syncytial virus (HRSV) are important etiologic agents of acute respiratory infections. In this study, a duplex polymerase chain reaction (PCR) assay was developed for the simultaneous detection of HAdV and HRSV in clinical samples. Sixty previously screened nasopharyngeal aspirates were used 20 HAdV-positive, 20 HRSV-positive and 20 double-negative controls. Eight samples were positive for both viruses. The duplex PCR assay proved to be as sensitive and specific as single-target assays and also detected the mixed infections with certainty. The identification of both viruses in a single reaction offers a reduction in both cost and laboratory diagnostic time.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Infecciones del Sistema Respiratorio
/
Adenoviridae
/
Nasofaringe
/
Virus Sincitial Respiratorio Humano
/
Infecciones por Virus Sincitial Respiratorio
/
Infecciones por Adenoviridae
Tipo de estudio:
Estudio diagnóstico
/
Estudio observacional
/
Estudio pronóstico
Límite:
Niño
/
Humanos
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2009
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Brasil
Institución/País de afiliación:
Instituto Nacional de Pesquisas da Amazônia/BR
/
Universidade de São Paulo/BR
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