Isla de patogenicidad de Vibrio parahaemolyticus en cepas chilenas clínicas y ambientales / Pathogenicity island region of clinical and environmental strains of Vibrio parahaemolyticus, isolated in Chile
Rev. méd. Chile
;
137(2): 208-214, feb. 2009. ilus, tab
Artículo
en Español
| LILACS
| ID: lil-516085
ABSTRACT
Background:
Most clinical isolates of Vibrio parahaemolyticus produce a major virulence factor known as the thermostable direct hemolysin (TDH). TDH is encoded by the tdh gene which is located in a genomic pathogenicity island (PAI). Most environmental isolates are described as tdh negative.Aim:
To assess if environmental strains lack the full pathogenicity island or if only the tdh gene is deleted. Material andmethods:
Thirty eight clinical and 66 environmental strains of Vibrio parahaemolyticus were studied. PAI was characterized by polymerase chain reaction (PCR). The presence of tdhA and tdhS genes, was determined by Southern blot.Results:
Fifty three environmental strains (80%) lacked a full PAI when compared with clinical strains. In environmental strains, Southern blot and sequence analysis showed that a genetic región of 80 kilobase pairs including genes from VPA1310 to VPA1396 was missing.Conclusions:
These results highlight the genetic dynamism of Vibrio parahaemolyticus pathogenecity island región and suggest that new pathogenic strains could appear by horizontal transfer of the island between toxigenic and non-toxigenic strains.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Vibrio parahaemolyticus
/
Islas Genómicas
/
Proteínas Hemolisinas
Límite:
Humanos
País/Región como asunto:
America del Sur
/
Chile
Idioma:
Español
Revista:
Rev. méd. Chile
Asunto de la revista:
Medicina
Año:
2009
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Chile
Institución/País de afiliación:
Universidad de Chile/CL
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