Conversion of barley SNPs into PCR-based markers using dCAPS method
Genet. mol. biol
;
32(3): 564-567, 2009. ilus, tab
Artículo
en Inglés
| LILACS
| ID: lil-522319
ABSTRACT
Molecular genetic research relies heavily on the ability to detect polymorphisms in DNA. Single nucleotide polymorphisms (SNPs) are the most frequent form of DNA variation in the genome. In combination with a PCR assay, the corresponding SNP can be analyzed as a derived cleaved amplified polymorphic sequence (dCAPS) marker. The dCAPS method exploits the well-known specificity of a restriction endonuclease for its recognition site and can be used to virtually detect any SNP. Here, we describe the use of the dCAPS method for detecting single-nucleotide changes by means of a barley EST, CK569932, PCR-based marker.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Hordeum
/
Polimorfismo de Nucleótido Simple
Idioma:
Inglés
Revista:
Genet. mol. biol
Asunto de la revista:
Genética
Año:
2009
Tipo del documento:
Artículo
País de afiliación:
Irán
/
Japón
Institución/País de afiliación:
Isfahan University of Technology/IR
/
National Institute of Agrobiological Sciences/JP
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