IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: cross-reaction with venoms from Bothrops species from Argentina
J. venom. anim. toxins incl. trop. dis
; J. venom. anim. toxins incl. trop. dis;15(3): 460-478, 2009. ilus, tab
Article
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| LILACS, VETINDEX
| ID: lil-525814
Biblioteca responsable:
BR68.1
ABSTRACT
We examined the ability of IgG anti-crotalic PLA2 to cross-react with Bothrops spp. venoms, from snakes found in the northeast of Argentina. Immunoblotting and ELISA tests showed that IgG anti-crotalic PLA2 recognize antigens of bothropic venoms. Indirect hemolytic activity tests showed that the quantity of antibodies that neutralized 50% of Crotalus durissus terrificus venom (ED50 2.1 mg IgG anti-crotalic PLA2/100 µg of venom) were also able to neutralize venom from other snakes in the following proportion 34% of B. alternatus, 18% of B. diporus and 12% of B. jararacussu. Likewise, direct PLA2 activity neutralization tests showed a similar cross-neutralization pattern including 56% of B. alternatus, 29% of B. diporus and 30% of B. jararacussu. In addition, in a myotoxic activity neutralization test, measured by plasma activity of creatine kinase, 35% of B. alternatus venom and 26% of B. diporus venom were neutralized, while no neutralization was detected with B. jararacussu venom. This study presents original data concerning cross-reactions between bothropic venoms from Argentina and IgG anti-crotalic PLA2. Our results suggest that anti-crotalic PLA2 antibodies should not be used to neutralize PLA2 activity of B. alternatus, B. diporus and especially B. jararacussu venoms; nor to enrich commercial antivenoms against these Bothrops species.(AU)
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Texto completo:
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Índice:
LILACS
Asunto principal:
Inmunoglobulina G
/
Pruebas de Neutralización
/
Fosfolipasas A2
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Anticuerpos
Límite:
Animals
País/Región como asunto:
America do sul
/
Argentina
Idioma:
En
Revista:
J. venom. anim. toxins incl. trop. dis
Asunto de la revista:
TOXICOLOGIA
Año:
2009
Tipo del documento:
Article