Use of a saline gradient for the diagnosis of schistosomiasis
Mem. Inst. Oswaldo Cruz
;
104(5): 720-723, Aug. 2009. ilus, graf
Artículo
en Inglés
| LILACS
| ID: lil-528080
ABSTRACT
The development of novel methods for parasitological diagnosis that are both highly sensitive and low in cost has been strongly recommended by the World Health Organization. In this study, a new technique for diagnosis of schistosomiasis mansoni is proposed based on the differential sedimentation of eggs when subjected to a slow continuous flux of 3 percent saline solution through a porous plaque. This influx suspends low-density faecal material, effectively cleaning the sample. The remaining sediment covering the porous plaque surface is then transferred to a glass slide and examined under a bright field microscope. Twelve Kato-Katz slides were used for comparison in the present study. Our results suggest that the saline gradient method detects a signifi-cantly higher number of eggs than the 12 Kato-Katz slides (p < 0.0001). We also found microscopic inspection to be quicker and easier with our newly described method. After cleaning the sample, the obtained sediment can also be conserved in a 10 percent formaldehyde solution and examined for at least 45 days later without statistically significant egg count differences.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Recuento de Huevos de Parásitos
/
Schistosoma mansoni
/
Esquistosomiasis mansoni
/
Cloruro de Sodio
/
Heces
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2009
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Fiocruz/BR
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