Chitinase production by Streptomyces sp. ANU 6277
Braz. j. microbiol
;
40(4): 725-733, Oct.-Dec. 2009. ilus, graf, tab
Artículo
en Inglés
| LILACS
| ID: lil-528154
ABSTRACT
Chitinase production by a terrestrial Streptomyces sp. ANU 6277 was studied under sub-merged fermentation. Chitinase production started after 24 h of incubation and reached maximum levels after 60 h of cultivation. A high level of chitinase activity was observed in the culture medium with pH 6 at 35ºC. Culture medium amended with 1 percent chitin was found to be suitable for maximum production of chitinase. An optimum concentration of colloidal chitin for chitinase production was determined. Studies on the influence of additional carbon and nitrogen sources on chitinase production revealed that starch and yeast extract served as good carbon and nitrogen sources to enhance chitinase yield.Chitinase was purified from crude enzyme extract by single step gel filtration by Sephadex G-100. Purified chitinase of the strain exhibited a distinct protein band near 45 kDa by means of SDS-PAGE.
Texto completo:
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Índice:
LILACS (Américas)
Asunto principal:
Streptomyces
/
Biosíntesis de Proteínas
/
Quitinasas
/
Medios de Cultivo
Idioma:
Inglés
Revista:
Braz. j. microbiol
Asunto de la revista:
Microbiologia
Año:
2009
Tipo del documento:
Artículo
País de afiliación:
India
Institución/País de afiliación:
Acharya Nagarjuna University/IN
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