New vectors derives from pUC 18 for clonig and thermal-induced expression in Escherichia coli
Braz. j. microbiol
;
40(4): 778-781, Oct.-Dec. 2009. ilus
Artículo
en Inglés
| LILACS
| ID: lil-528159
ABSTRACT
We report the construction of two vectors for Escherichia coli pUC72, for molecular cloning, and pPLT7, for thermal-induced expression. The main feature of pUC72 is a novel polylinker region that includes restriction sites for Nde I and Nco I which provide an ATG codon for proper translation initiation of expressed genes. Vector pPLT7 is ideal for thermo-inducible expression in host cells that carry the cI857 repressor gene. The use of pPLT7 was validated by the successful expression of the genes encoding carp and porcine growth hormones. These vectors provide novel cloning possibilities in addition to simple, non-expensive, high level expression of recombinant proteins in E. coli.
Texto completo:
Disponible
Índice:
LILACS (Américas)
Asunto principal:
Técnicas In Vitro
/
Secuencia de Bases
/
Proteínas
/
Expresión Génica
/
Clonación Molecular
/
Escherichia coli
/
Fragmentación del ADN
Idioma:
Inglés
Revista:
Braz. j. microbiol
Asunto de la revista:
Microbiologia
Año:
2009
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Universidade Federal de Goiás/BR
/
Universidade Federal do Amazonas/BR
/
Universidade de Brasília/BR
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