Glass bead transformation method for gram-positive bacteria

Rattanachaikunsopon, Pongsak; Phumkhachorn, Parichat

Rattanachaikunsopon, Pongsak; Phumkhachorn, Parichat.

Rattanachaikunsopon, Pongsak; Ubon Ratchathani University. Department of Biological Science. Ubon Ratchathani. TH
Phumkhachorn, Parichat; Ubon Ratchathani University. Department of Biological Science. Ubon Ratchathani. TH

Braz. j. microbiol ; 40(4): 923-926, Oct.-Dec. 2009. graf, tab

Artículo en Inglés | LILACS | ID: lil-528176

ABSTRACT

ABSTRACT

A simple, inexpensive and reproducible transformation method was developed for Gram-positive bacteria. It was based on agitation of bacterial protoplasts with glass beads in the presence of DNA and polyethylene glycol. By using this method, introduction of pGK12 into protoplasts of several strains of Gram-positive bacteria was achieved.

Asunto(s)

Bacterias Grampositivas/genética; Genética Microbiana; Protoplastos; Transformación Bacteriana; Vidrio/análisis; Métodos; Métodos

Glass bead; Gram-positive bacteria; Transformation

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Texto completo: Disponible Índice: LILACS (Américas) Asunto principal: Protoplastos / Transformación Bacteriana / Genética Microbiana / Vidrio / Bacterias Grampositivas Idioma: Inglés Revista: Braz. j. microbiol Asunto de la revista: Microbiologia Año: 2009 Tipo del documento: Artículo País de afiliación: Tailandia Institución/País de afiliación: Ubon Ratchathani University/TH

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